EasyBlot anti-Sheep IgG Kit (Optimized for Prot A/G)

Catalogue Number: GTX228911-01-GTX

Manufacturer:	GeneTex
Preservative:	0.005% Thimerosal
Type:	IHC kits
Shipping Condition:	Dry Ice
Unit(s):	250 ul, 50 ul
Host name:
Clone:
Isotype:
Immunogen:
Application:	WB

Overview
Datasheets

Additional Text

Purification

Protein G purified

Concentration

0.18 mg/ml

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

Suggested starting dilutions for WB is 1:1000. Use 2-5 µg of the IP antibody; do not exceed 5 µg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Short Description

EasyBlot anti-Sheep IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of IgG and does not bind to the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). The protein A/G beads used in the IP procedure sometimes bind to IgG and produce contaminating signals at approximately 50 kDa and 25 kDa. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex's EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX228911-01 Datasheet

Price

£349.00

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