PSMB6 antibody
Catalogue Number: GTX23331-GTX
GTX23331 Image
GTX23331 Image
GTX23331 Image
GTX23331 Image
GTX23331 Image
GTX23331 Image
WB analysis of 25 ug of Hela (lane 1) and BAEC (lane 2) cell lysates using GTX23331 PSMB6 antibody. Dilution : 1:1000
GTX23331 Image
ICC/IF analysis of 3T3 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 5 µg/ml for 1 hour at room temperature
GTX23331 Image
ICC/IF analysis of A431 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4C
GTX23331 Image
ICC/IF analysis of BAEC cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4C
GTX23331 Image
ICC/IF analysis of Hela cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4C
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | proteasome (prosome, macropain) subunit, beta type 6 , Lmp19 , Mpnd |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58) |
| Application: | ICC, IF, WB |
Description
Description: Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
Additional Text
Concentration
1 mg/ml
Gene Name
PSMB6
Purification
Affinity Purified
Gene ID
19175
Uniprot ID
Q60692
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
25
Application Notes
WB: 1 µg/ml. ICC/IF: 1:10-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX23331 Datasheet
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