NFAT5 antibody
Catalogue Number: GTX23446-GTX
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IHC-P analysis of human brain tissue using GTX23446 NFAT5 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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IHC-P analysis of human skeletal muscle tissue using GTX23446 NFAT5 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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IP analysis of U2OS cell lysates using GTX23446 NFAT5 antibody. IP reaction : 3µg antibody / 500µg lysate
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WB analysis of 25 ug of various whole cell lysates using GTX23446 NFAT5 antibody. Dilution : 1:1000
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ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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ICC/IF analysis of MCF-7 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of NIH-3T3 cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX23446 NFAT5 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 166 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | nuclear factor of activated T cells 5 , NF-AT5 , NFATL1 , NFATZ , OREBP , TONEBP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC, EMSA |
Description
Description: The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
10725
Gene Name
NFAT5
Uniprot ID
O94916
Purification
Affinity Purified
Molecular Weight
166
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1,000. ICC/IF: 1:100-1:1000. IHC-P: 1:20. IP: 3 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX23446 Datasheet
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