SIN3A antibody
Catalogue Number: GTX23479-GTX
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IHC-P analysis of human ovary carcinoma tissue using GTX23479 SIN3A antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
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WB analysis of K562 cell extract using GTX23479 SIN3A antibody.
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ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. IP reaction : 1.0ul antibody / 100ul sample
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ICC/IF analysis of A431 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
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ICC/IF analysis of Hela cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4oC
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ICC/IF analysis of NIH-3T3 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 145 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SIN3 transcription regulator family member A , WITKOS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
SIN3A
Uniprot ID
Q96ST3
Purification
Affinity Purified
Gene ID
25942
Molecular Weight
145
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 0.5 µg/ml. ICC/IF: 1:50-1:500. IHC-P: 1:50-1:500. ChIP assay: 1-3 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX23479 Datasheet
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