PPAR alpha (phospho Ser12) antibody
Catalogue Number: GTX23484-GTX
GTX23484 Image
GTX23484 Image
GTX23484 Image
GTX23484 Image
GTX23484 Image
GTX23484 Image
WB analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates using GTX23484 PPAR alpha (phospho Ser12) antibody. Dilution : 1:200
GTX23484 Image
WB analysis of whole cell extracts (20 µg lysate) of U-87 MG (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), C2C12 (lane 4), Hep G2 (lane 5) and NIH/3T3 (lane 6) using GTX23484 PPAR alpha (phospho Ser12) antibody. Dilution : 1:250-1:1000
GTX23484 Image
ICC/IF analysis of 3T3-L1 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX23484 Image
ICC/IF analysis of C2C12 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX23484 Image
ICC/IF analysis of U-87 MG cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 52 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | peroxisome proliferator activated receptor alpha , 4933429D07Rik , AW742785 , Nr1c1 , PPAR-alpha , PPARalpha , Ppar |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha. |
| Application: | FC, ICC, IF, WB, EMSA |
Description
Description: Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the phospho-PPAR alpha is NR1C1.
Additional Text
Gene Name
PPARA
Uniprot ID
P23204
Purification
Affinity Purified
Modification
Phosphorylated, Ser12
Molecular Weight
52
Gene ID
19013
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:100-1:1000. ICC/IF: 1:100-1:500. FCM: 3-5 µg/10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX23484 Datasheet
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