MCM3 antibody
Catalogue Number: GTX24460-GTX
GTX24460 Image
GTX24460 Image
GTX24460 Image
GTX24460 Image
GTX24460 Image
WB analysis of HeLa and 293T cell lysate prepared by NETN lysis buffer using GTX24460 MCM3 antibody. Dilution : 0.1 µg/ml Loading : 50µg
GTX24460 Image
WB analysis of HeLa cell lysate in RIPA buffer using GTX24460 MCM3 antibody. Loading : 50µg Dilution : 0.2 µg/ml
GTX24460 Image
IHC-P analysis of human colon carcinoma tissue using GTX24460 MCM3 antibody. Dilution : 1 µg/ml
GTX24460 Image
PLA analysis of MCF-7 cells using GTX24460 MCM3 antibody and anti-human POLR2A antibody. Green : Actin Red : positive signal Blue : DAPI
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 91 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | minichromosome maintenance complex component 3 , HCC5 , P1-MCM3 , P1.h , RLFB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172). |
| Application: | IHC-P, IP, WB, PLA |
Description
Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]
Additional Text
Gene Name
MCM3
Uniprot ID
P25205
Gene ID
4172
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
91
Concentration
1 mg/ml
Application Notes
WB: 1:2000 - 1:10000. IHC-P: 1:1000 - 1:5000. IP: 2 - 5 µg/mg lysate. PLA: 1:2000 - 1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE.
GTX24460 Datasheet
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