Tau (phospho Ser199) antibody
Catalogue Number: GTX24749-GTX
GTX24749 Image
GTX24749 Image
GTX24749 Image
GTX24749 Image
GTX24749 Image
IHC-P analysis of human brain tissue using GTX24749 Tau (phospho Ser199) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
GTX24749 Image
IHC-P analysis of mouse brain tissue using GTX24749 Tau (phospho Ser199) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
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WB (peptide competition) analysis of human recombinant Tau untreated (Lane 1) or treated with GSK-3 beta (1 µg per µg Tau) for 45 minutes (Lanes 2-5) using GTX24749 Tau (phospho Ser199) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
GTX24749 Image
ICC/IF analysis of SH-SY5Y cells using GTX24749 Tau (phospho Ser199) antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton? X-100 for 10 minutes
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat. |
| Application: | FACS, ICC, IF, IHC-P, WB, IHC, FA, IHC-Flt |
Description
Description: This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
MAPT
Gene ID
4137
Uniprot ID
P10636
Purification
Affinity Purified
Molecular Weight
79
Modification
Phosphorylated, Ser199
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
ICC/IF: 1µg/mL. IHC-P: 1:20-1:200. FACS: 3-5 µg/million cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX24749 Datasheet
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