ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody
Catalogue Number: GTX24819-GTX
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GTX24819 Image
GTX24819 Image
GTX24819 Image
GTX24819 Image
GTX24819 Image
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IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:50
GTX24819 Image
IHC-P analysis of human colon carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
GTX24819 Image
IHC-P analysis of mouse stomach tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
GTX24819 Image
WB analysis of NIH3T3 untreated (Lane 1) or treated with PDGF (Lane 2) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Loading: 5 ug
GTX24819 Image
WB (peptide competition) analysis of PC12 cells stimulated with 0.5 M sorbitol for 5 minutes (Lane 2-6) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
GTX24819 Image
WB analysis of whole cell extracts (30 µg lysate) of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly. |
| Application: | ICC, IF, IHC-P, WB, IHC, IHC-Flt |
Description
Description: kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]
Additional Text
Purification
Affinity Purified
Modification
Phosphorylated, Thr185
Uniprot ID
P27361, P28482
Gene ID
5594; 5595
Gene Name
MAPK1, MAPK3
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000. IHC-P: 1:10-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity
mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX24819 Datasheet
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