Hsp70 antibody [4G4]
Catalogue Number: GTX25444-GTX
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IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
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IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4]. IP reaction : 2µg antibody / 500µg lysate Dilution : 1:1000
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WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4]. Dilution : 1:1000
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ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 C
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ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 C
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ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 C
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | 4G4 |
| Isotype: | IgG1 |
| Immunogen: | Human recombinant HSP70 over expressed in E. coli. |
| Application: | FACS, ICC, IF, IHC-P, IP, WB, IHC, NT, Inh, CTA, IM |
Additional Text
Gene ID
3303; 3304
Purification
Unpurified
Uniprot ID
P0DMV8, P0DMV9
Gene Name
HSPA1A, HSPA1B
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Specificity
heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70
Application Notes
WB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:20 - 50. FACS: 1:100 - 1:200. IP: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX25444 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations