Thyroid Hormone Receptor alpha antibody
Catalogue Number: GTX25621-GTX
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IHC-P analysis of human skin tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
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IHC-P analysis of human colon carcinoma tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:500
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IHC-P analysis of human thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:500
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IHC-P analysis of rat thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
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IHC-P analysis of human thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
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IHC-P analysis of mouse thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
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WB analysis of 25 ug of A431 (lane 1), rat thyroid (lane 2) and mouse thyroid (lane 3) cell lysates using GTX25621 Thyroid Hormone Receptor alpha antibody. Dilution : 1:500
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ICC/IF analysis of A431 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 C
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ICC/IF analysis of L6 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 C
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ICC/IF analysis of U251 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 C
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | thyroid hormone receptor alpha , AR7 , CHNG6 , EAR7 , ERB-T-1 , ERBA , ERBA1 , NR1A1 , THRA1 , THRA2 , c-ERBA-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic Peptide: E(403) V F E D Q E V(410) |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC, EMSA |
Description
Description: The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
7067
Gene Name
THRA
Uniprot ID
P10827
Molecular Weight
55
Purification
Unpurified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:200. ICC/IF: 1:10-1:200. IHC-P: 1:20-1:100. Gel supershift assays: 1:10. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX25621 Datasheet
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