PKA beta (catalytic subunit) (phospho Ser338) antibody
Catalogue Number: GTX25816-GTX
GTX25816 Image
GTX25816 Image
GTX25816 Image
ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 µg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene. IP reaction : 1.0ul antibody / 100ul sample
GTX25816 Image
WB (peptide competition) analysis of 3T3-L1 cells using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 41 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | protein kinase cAMP-activated catalytic subunit beta , PKA C-beta , PKACB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKA catalytic b subunit that contains serine 338. The sequence is conserved in cow and pig. |
| Application: | ChIP, WB |
Description
Description: The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
Additional Text
Gene ID
5567
Gene Name
PRKACB
Uniprot ID
P22694
Purification
Affinity Purified
Modification
Phosphorylated, Ser338
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
41
Application Notes
ChIP assay: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX25816 Datasheet
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