GLI3 antibody
Catalogue Number: GTX26050-GTX
GTX26050 Image
GTX26050 Image
GTX26050 Image
GTX26050 Image
GTX26050 Image
GTX26050 Image
GTX26050 Image
Immunohistochemistry of Rabbit anti-Gli-3 antibody (GTX26050). This image tissue: human glioblastoma. Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Gli-3 antibody at 0.625 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear and smooth muscle. Staining: Gli-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
GTX26050 Image
Western Blot of Rabbit anti-Gli-3 antibody. Lane 1: 50 kDa molecular weight marker. Lane 2: 293T cells transfected with CrkL-Flag. Lane 3: 293T cells transfected with human Gli-3. Load: 35 µg per lane. Primary antibody: Gli-3 antibody at 1:400 for overnight at 4oC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4oC. Predicted/Observed size: 170-190 kDa for hGli-3. Other band(s): Non specific background ~60kDa.
GTX26050 Image
ICC/IF analysis of MCF-7 cells using GTX26050 GLI3 antibody. Red : Primary antibody Green : alpha Tubulin Dilution : 5 µg/mL
GTX26050 Image
IHC-P analysis of human glioblastoma tissue using GTX26050 GLI3 antibody. Dilution : 0.625 µg/mL
GTX26050 Image
WB analysis of non-transfected and transfected 293T whole cell lysate using GTX26050 GLI3 antibody. Loading : 35 µg Dilution : 1:400
GTX26050 Image
WB analysis of various samples using GTX26050 GLI3 antibody. Lane 1 : Human brain tissue lysate Lane 2 : Human lung tissue lysate Lane 3 : Human spleen tissue lysate Lane 4 : Mouse brain tissue lysate Lane 5 : Mouse lung tissue lysate Loading : 20 µg Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 170 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GLI family zinc finger 3 , ACLS , GCPS , GLI3-190 , GLI3FL , PAP-A , PAPA , PAPA1 , PAPB , PHS , PPDIV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein. |
| Application: | ELISA, ICC, IF, IHC-P, WB, Multiplex |
Description
Description: Gli3 is belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.
Additional Text
Gene Name
GLI3
Uniprot ID
P10071
Gene ID
2737
Purification
Affinity Purified
Molecular Weight
170
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:2000. IHC-P: 0.5-5 µg/mL. ELISA: 1:6000-1:30000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX26050 Datasheet
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