Collagen I antibody (Biotin)
Catalogue Number: GTX26577-GTX
GTX26577 Image
GTX26577 Image
GTX26577 Image
GTX26577 Image
GTX26577 Image
GTX26577 Image
GTX26577 Image
GTX26577 Image
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Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody. Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.
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Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B)
GTX26577 Image
Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B)
GTX26577 Image
Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody at 5µg/mL for 45 min at 4oC. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.
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Western Blot of Rabbit anti-Collagen I antibody (GTX26577). Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.2-2 µg/10 ml for overnight at 4oC. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4oC. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
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IHC-P analysis of human skin tissue at pH 6 using GTX26577 Collagen I antibody (Biotin). Panel A : Positive control Panel B : Negative control Dilution : 10 µgm/mL
GTX26577 Image
IHC-P analysis of human skin tissue at pH 9 using GTX26577 Collagen I antibody (Biotin). Panel A : Positive control Panel B : Negative control Dilution : 10 µg/mL
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WB analysis of Wistar rat hepatic stellate whole cell lysate using GTX26577 Collagen I antibody (Biotin). Left lane : GFP-transduced Right lane : PPARg-transduced Loading : 100 µg
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 139 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Collagen Type I from human and bovine placenta. |
| Application: | FC, ICC, IF, IHC-P, WB, DB, Multiplex |
Description
Description: This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Additional Text
Gene Name
COL1A1
Uniprot ID
P02452
Purification
Affinity Purified
Gene ID
1277
Molecular Weight
139
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:3000-1:6000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX26577 Datasheet
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