GFP antibody (FITC)
Catalogue Number: GTX26662-GTX
GTX26662 Image
GTX26662 Image
GTX26662 Image
Immunofluorescence Microscopy of GFP-GOAT-Antibody. Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP (GTX26662) was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
GTX26662 Image
Western Blot of Goat anti-GFP Fluorescein Conjugated Antibody (GTX26662). Lane 1: GFP. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Predicted/Observed size: 28 kDa, 33 kDa for GFP. Other band(s): None.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Application: | ELISA, ICC, IF, WB, IHC-Fr, IHC, IHC-WM |
Description
Description: The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.
Additional Text
Concentration
1 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: >1:10000. ICC/IF: 1:500 - 1:2500. ELISA: >1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX26662 Datasheet
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