GFP antibody (HRP)
Catalogue Number: GTX26663-GTX
GTX26663 Image
GTX26663 Image
GTX26663 Image
Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20oC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
GTX26663 Image
WB analysis of various samples using GTX26663 GFP antibody (HRP). Lane 1 and 14 : Protein ladder Lane 2 : 293T whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : CHO-K1 whole cell lysate Lane 5 : C2C12 whole cell lysate Lane 6 : NIH-3T3 whole cell lysate Lane 7 : Mouse embryonic fibroblast lysate Lane 8 : HCP Lane 9 : MBP Lane 10 : RFP Lane 11 : GFP Lane 12 : GST Lane 13 : MBP Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Gentamicin sulfate |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Application: | ELISA, ICC, IF, IP, WB, IHC, Multiplex |
Description
Description: Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:2000-1:5000. ELISA: 1:10000-1:50000. IHC: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX26663 Datasheet
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