IL6 antibody
Catalogue Number: GTX26672-GTX
GTX26672 Image
GTX26672 Image
GTX26672 Image
Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 µg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.
GTX26672 Image
WB analysis of recombinant human IL-6 protein using GTX26672 IL6 antibody. Lane 1 : Protein ladder Lane 2 : 50 ng Lane 3 : 20 ng Lane 4 : 10 ng Lane 5 : Mock Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 24 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant human IL-6 produced in E.coli. |
| Application: | ELISA, IHC-P, WB, IHC-Fr, DB, NT, Inh |
Description
Description: Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important asIL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.
Additional Text
Gene Name
IL6
Gene ID
3569
Molecular Weight
24
Uniprot ID
P05231
Concentration
80 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000. IHC-P: 1:1:100-1:200. ELISA: 1:500-1:2000. Neutralizing/Inhibition: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX26672 Datasheet
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