MGMT antibody [MT 23.2]
Catalogue Number: GTX27045-GTX
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WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000
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WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].
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ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Blue : Hoechst 33258 Dilution : 1:50
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ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:200 Fixation : 10% formalin Permibilization : 0.5% Triton-X100
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IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:2000 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
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FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 1 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 2 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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The data was published in the journal Oncotarget in 2016. PMID: 27057640
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 22 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | O-6-methylguanine-DNA methyltransferase |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | MT 23.2 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455] |
| Application: | FC, ICC, IF, IHC-P, WB, MS |
Additional Text
Gene Name
MGMT
Uniprot ID
P16455
Gene ID
4255
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
22
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:1000. ICC/IF: 1:500. IHC-P: 1:500 - 1:2000. FCM: 1 - 2 µg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX27045 Datasheet
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