ABCA1 antibody
Catalogue Number: GTX27360-GTX
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GTX27360 Image
GTX27360 Image
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WB analysis of mouse peritoneal macrophages using GTX27360 ABCA1 antibody. Lane 1 : T09 cell lysate Lane 2 : Induced T09 cell lysate
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WB analysis of post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol using GTX27360 ABCA1 antibody. Loading : 40µg
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WB analysis of RAW264.7 cells treated with vehicle (-) or 9-cisretinoic acid and 22rhydroxycholesterol (+) using GTX27360 ABCA1 antibody. Loading : 40µg
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IHC-P analysis of prostate tissue using GTX27360 ABCA1 antibody.
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ICC/IF analysis of HepG2 cells using GTX27360 ABCA1 antibody. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 5 µg/ml Fixation : 10% formalin Permibilization : 0.5% Triton-X100
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ICC/IF analysis of HepG2 cells(serum starvation 24hrs and treated with 1uM TO9) using GTX27360 ABCA1 antibody. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 5 µg/ml Fixation : 10% formalin Permibilization : 1X TBS + 0.5% Triton-X100 for 5 minutes
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FACS analysis of HeLa cells using GTX27360 ABCA1 antibody. M1 is defined by unstained cells. Purple : primary antibody Dilution : 1:400
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 254 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ATP binding cassette subfamily A member 1 , ABC-1 , ABC1 , CERP , HDLDT1 , TGD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, EMSA |
Description
Description: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq]
Additional Text
Gene Name
ABCA1
Gene ID
19
Purification
Affinity Purified
Molecular Weight
254
Uniprot ID
O95477
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500. ICC/IF: 1:100. IHC-P: 1:200. FCM: 1:400. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX27360 Datasheet
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