Streptavidin (HRP)
Catalogue Number: GTX27403-GTX
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GTX27403 Image
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Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)
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Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)
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Dot blot analysis of BSA with (row A) and without (row B) biotin conjugation using GTX27403 Streptavidin (HRP). Lane 1 : 100 ng Lane 2 : 33.33 ng Lane 3 : 11.11 ng Lane 4 : 3.7 ng Lane 5 : 1.23 ng Dilution : 1 µg/mL
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WB analysis of human IL-7 protein using biontin-conjugated anti-IL-7 antibody followed by GTX27403 Streptavidin (HRP). Loading : 50 ng Dilution : 1:40000
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WB analysis of GST protein using biotin-conjugated anti-GST antibody followed by GTX27403 Streptavidin (HRP). Loading : 0.05 µg Dilution : 1:40000
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WB analysis of human IL-7 protein using Human IL-7 Biotin conjugated antibody followed by GTX27403 Streptavidin (HRP). Loading : 50 ng Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Avidin/Streptavidin Biotin Products |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Application: | ELISA, IHC-P, WB, IHC-Fr, DB, IM |
Additional Text
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:10,000-1:40,000. IHC-P: 1:1,000-1:5,000. ELISA: 1:20,000-1:200,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX27403 Datasheet
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