RFX5 antibody
Catalogue Number: GTX27542-GTX
GTX27542 Image
GTX27542 Image
GTX27542 Image
GTX27542 Image
Western Blot of Rabbit Anti-RFX5 antibody (GTX27542). Raji B cell nuclear extract lysates. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4oC. Secondary antibody: HRP Goat-a-Rabbit IgG at 1:5,000 for 45 min at RT.
GTX27542 Image
WB analysis of Raji nuclear extract using GTX27542 RFX5 antibody. Loading : 35 µg Dilution : 1:2500
GTX27542 Image
WB analysis of HeLa nuclear extract using GTX27542 RFX5 antibody. Loading : 25 µg Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 65 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | regulatory factor X5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to KLH. |
| Application: | ELISA, ICC, IF, IP, ChIP, WB, EMSA |
Description
Description: RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex, which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.
Additional Text
Gene Name
RFX5
Gene ID
5993
Molecular Weight
65
Uniprot ID
P48382
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000. ELISA: 1:5000-1:25000. EMSA: 0.5-1 µL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX27542 Datasheet
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