IKB alpha antibody

Catalogue Number: GTX27545-GTX

Manufacturer:	GeneTex
Preservative:	0.1% Sodium azide
Molecular Weight:	36
Physical state:	Liquid
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI
Shipping Condition:	Blue Ice
Unit(s):	50 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Synthetic IkBa peptide corresponding to a region near the C-terminus of the human protein.
Application:	ELISA, WB, IHC, EMSA

Overview
Datasheets

Description

Description: Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.

Additional Text

Gene ID

4792

Gene Name

NFKBIA

Uniprot ID

P25963

Concentration

80 mg/ml

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Molecular Weight

Application Notes

WB: 1:2000-1:10000. ELISA: 1:20000-1:100000. IHC: 1:1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX27545 Datasheet

GTX27545 Reference

Price

£410.00

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