GAPDH antibody [6C5]
Catalogue Number: GTX28245-GTX
GTX28245 Image
GTX28245 Image
GTX28245 Image
GTX28245 Image
Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by: GTX28245 (green colour) F-actin microfilaments-bindingdye (red) DNA-binding dye (dark blue)
GTX28245 Image
Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A). Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 oC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. Lane 1: Human GAPDH (1 µg) Lane 2: GAPDH immunoprecipitated from rat heart tissue extract Lane 3: Only GTX28245 preincubated with Protein A Sepharose Lane 4: Only Protein A Sepharose
GTX28245 Image
Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded. Lane 1: isolated human GAPDH, 0.5 µg Lane 2: human heart tissue extract Lane 3: pig heart tissue extract Lane 4: goat heart tissue extract Lane 5: bovine heart tissue extract Lane 6: dog heart tissue extract Lane 7: mouse heart tissue extract Lane 8: rat heart tissue extract Lane 9: rabbit heart tissue extract
| Manufacturer: | GeneTex |
| Preservative: | 0.09% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 6C5 |
| Isotype: | IgG1 |
| Immunogen: | Rabbit muscle GAPDH |
| Application: | ICC, IF, IP, WB |
Additional Text
Gene Name
GAPDH
Gene ID
100009074
Purification
Protein A purified
Uniprot ID
P46406
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
36
GTX28245 Datasheet
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