Angiopoietin 2 antibody
Catalogue Number: GTX28452-GTX
GTX28452 Image
GTX28452 Image
GTX28452 Image
GTX28452 Image
GTX28452 Image
IHC-P of anti-ANG2 Antibody (GTX28452) in human placenta tissue. Antigen Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml for 1.5 hour RT.
GTX28452 Image
Western blotting. Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants of mouse-angiopoietin-expressing endothelial cells. Lane 1 : mock endothelial cells. Lane 2 : mouse Ang-2 expressing cells. Lane 3 - mouse Ang-1 expressing cells. Lane 4 - mouse Ang-1 expressing cells. Lane 5 - wt endothelial cells. Approximately 20 µg of each lysate was loaded for 10% SDS-PAGE.
GTX28452 Image
IHC-P analysis of human placenta tissue using GTX28452 Angiopoietin 2 antibody. Antigen retrieval : Head mediated citrate buffer (pH 6.2) Dilution : 2 µg/mL
GTX28452 Image
WB analysis of mouse-angiopoietin-expressing endothelial whole cell lysate using GTX28452 Angiopoietin 2 antibody. Lane 1 : Mock Lane 2 : Mouse Arg2 expressing whole cell lysate Lane 3 : Mouse Arg1 (clone 1-15) expressing whole cell lysate Lane 4 : Mouse Arg1 (clone 1-8) expression whole cell lysate Lane 5 : Wild type Loading : 20 µg Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Molecular Weight: | 57 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | angiopoietin 2 , Agpt2 , Ang-2 , Ang2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide. |
| Application: | IHC-P, IP, WB, Multiplex |
Description
Description: Angiopoietin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-1 antibody, GT28451) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.
Additional Text
Gene Name
ANGPT2
Uniprot ID
O35608
Concentration
80 mg/ml
Molecular Weight
57
Gene ID
11601
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:2000. IHC-P: 1:200-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX28452 Datasheet
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