NOTCH1 antibody
Catalogue Number: GTX28925-GTX
GTX28925 Image
GTX28925 Image
GTX28925 Image
GTX28925 Image
GTX28925 Image
GTX28925 Image
IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
GTX28925 Image
Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
GTX28925 Image
Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17s-estradiol. Lane 3: MCF-7 + 10 µM gamma secretase inhibitor. Load: 35 µg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4oC. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.
GTX28925 Image
Dot blot analysis of Notch1 N-terminal (row 1) and intra (row 2) peptides using GTX28925 NOTCH1 antibody. Lane 1 : 200 ng Lane 2 : 66.67 ng Lane 3 : 22.22 ng Lane 4 : 7.41 ng Lane 5 : 2.47 ng Dilution : 1:1000
GTX28925 Image
IHC-P analysis of human pancreas exocrine glands tissue using GTX28925 NOTCH1 antibody. Dilution : 1:200
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Molecular Weight: | 273 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, DB, Multiplex, NT, Inh |
Description
Description: Notch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Additional Text
Gene Name
NOTCH1
Uniprot ID
P46531
Gene ID
4851
Concentration
90 mg/ml
Molecular Weight
273
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:2000. IHC-P: 1:200. ELISA: 1:20000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX28925 Datasheet
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