NOTCH2 antibody
Catalogue Number: GTX28927-GTX
GTX28927 Image
GTX28927 Image
GTX28927 Image
GTX28927 Image
IHC-P analysis of human kidney tissue using Notch 2 antibody at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
GTX28927 Image
IHC-P analysis of human kidney tissue using GTX28927 NOTCH2 antibody. Dilution : 1:500
GTX28927 Image
WB analysis of Non-transfected (lane 4-6) and transfected (lane 1-3) SV40 whole cell lysate using GTX28927 NOTCH2 antibody. Lane 1 and 3 : Lysate 24 hr post transfection Lane 2 and 5 : Lysate 48 hr post transfection Lane 3 and 6 : Lysate 72 hr post transfection Loading : 100 µg Dilution : 1:400
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Molecular Weight: | 265 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | notch receptor 2 , AGS2 , HJCYS , hN2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). |
| Application: | ELISA, ICC, IF, IHC-P, WB, Multiplex |
Description
Description: Synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Additional Text
Gene ID
4853
Gene Name
NOTCH2
Uniprot ID
Q04721
Concentration
75 mg/ml
Molecular Weight
265
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:400-1:2000. IHC-P: 1:200-1:800. ELISA: 1:30000-1:90000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX28927 Datasheet
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