M-CSF antibody

Catalogue Number: GTX29693-GTX

Manufacturer:GeneTex
Preservative:no preservative|No preservative
Physical state:Liquid
Type:Polyclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 50 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: Highly pure (>98%) recombinant hM-CSF (human M-CSF)
Application: ELISA, WB, NT, Inh

Description

Description: Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including fibroblasts, macrophages and endothelial cells. This colony stimulation factor is involved in both the development and function of monocytes/macrophages.

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Application Notes

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C.