Catalogue Number: GTX29693-GTX
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Highly pure (>98%) recombinant hM-CSF (human M-CSF) |
| Application: | ELISA, WB, NT, Inh |
Description: Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including fibroblasts, macrophages and endothelial cells. This colony stimulation factor is involved in both the development and function of monocytes/macrophages.
Affinity Purified
Polyclonal
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
1 mg/ml
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required. ELISA:To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot:To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C.