EasyBlot Sampler Kit

Catalogue Number: GTX300111-GTX

Manufacturer:	GeneTex
Physical state:	Liquid
Type:	IHC kits
Shipping Condition:	Blue Ice
Unit(s):	1 kit

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Datasheets

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

Suggested starting dilutions for WB is 1:1000. Use 2-5 µg of the IP antibody; do not exceed 5 µg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Short Description

EasyBlot anti-Rabbit/Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit/Mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments.Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to mouse or rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTex's EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.

Storage Note

Please refer to each individual component datasheet for storage instructions.

GTX300111 Datasheet

Price

£120.00

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