HIF1 alpha antibody [H1alpha67]
Catalogue Number: GTX30105-GTX
GTX30105 Image
GTX30105 Image
GTX30105 Image
GTX30105 Image
GTX30105 Image
WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67]. Loading : 50µg
GTX30105 Image
ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67]. Red : primary antibody Blue : DAPI Dilution : 1 µg/ml
GTX30105 Image
IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
GTX30105 Image
IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 93 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | H1alpha67 |
| Isotype: | IgG2b |
| Immunogen: | A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665]. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, IHC, EMSA, Act |
Description
Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Additional Text
Gene Name
HIF1A
Gene ID
3091
Uniprot ID
Q16665
Purification
Protein G purified
Molecular Weight
93
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500. ICC/IF: 1:50. IHC-P: 1:20 - 1:50. IHC-Fr: 1:20 - 1:50. FCM: 1:10 - 1:1000. IP: 1:10 - 1:500. ELISA: 1:100 - 1:2000. ChIP assay: 1 - 5 µg. Gel supershift assays: 1:1 - 1:100. IHC: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX30105 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations