HIF1 alpha antibody [H1alpha67]
Catalogue Number: GTX30105-GTX
GTX30105 Image
GTX30105 Image
GTX30105 Image
GTX30105 Image
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WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67]. Loading : 50µg
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ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67]. Red : primary antibody Blue : DAPI Dilution : 1 µg/ml
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IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
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IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Alias: | hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | H1alpha67 |
| Isotype: | IgG2b |
| Immunogen: | A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665]. |
| Application: | ELISA, FACS, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, IHC, EMSA, Act |
Description
Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Additional Text
Gene Name
HIF1A
Concentration
1 mg/ml
Gene ID
3091
Uniprot ID
Q16665
Purification
Protein G purified
Molecular Weight
93
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500. ICC/IF: 1:50. IHC-P: 1:20 - 1:50. IHC-Fr: 1:20 - 1:50. FACS: 1:10 - 1:1000. IP: 1:10 - 1:500. ELISA: 1:100 - 1:2000. ChIP assay: 1 - 5 µg. Gel supershift assays: 1:1 - 1:100. IHC: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX30105 Datasheet
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