Catalogue Number: GTX30105-GTX
Manufacturer: | GeneTex |
Preservative: | 0.05% Sodium azide |
Physical state: | Liquid |
Type: | ChIP Validated Antibodies |
Alias: | hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78 |
Shipping Condition: | Blue Ice |
Unit(s): | 100 ul |
Host name: | Mouse |
Clone: | H1alpha67 |
Isotype: | IgG2b |
Immunogen: | A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665]. |
Application: | ELISA, FACS, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, IHC, EMSA, Act |
Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
HIF1A
1 mg/ml
3091
Q16665
Protein G purified
93
Monoclonal
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
WB: 1:500. ICC/IF: 1:50. IHC-P: 1:20 - 1:50. IHC-Fr: 1:20 - 1:50. FACS: 1:10 - 1:1000. IP: 1:10 - 1:500. ELISA: 1:100 - 1:2000. ChIP assay: 1 - 5 µg. Gel supershift assays: 1:1 - 1:100. IHC: 1:20 - 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.