HIF2 alpha antibody
Catalogue Number: GTX30114-GTX
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HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200. Blue: Hoechst 33342 staining.
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HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200. Blue: Hoechst 33342 staining.
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HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody (GTX30114) - Detection of HIF-2 Alpha (Green) in RCC4 cells using GTX30114. Nuclei (Blue) are counterstained with Hoechst 33258.
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Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes
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Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.
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Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114
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GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure.
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The data was published in the journal Cell Death Dis in 2014. PMID: 25010988
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The data was published in the journal Oncotarget in 2017. PMID: 29137374
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 97 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A peptide derived from the mouse/human HIF2 alpha. |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)
Additional Text
Gene Name
EPAS1
Uniprot ID
P97481
Purification
Affinity Purified
Gene ID
13819
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
97
Concentration
1 mg/ml
Application Notes
Recommended Starting Dilutions:For WB: Use at a dilution of 1:100-1:3000. Recognizes a band at 118kDa.For IHC: Use at a dilution of 1:100. For ICC/IF: Use at a dilution of 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.
Short Description
HIF2 alpha antibody recognizes HIF2 alpha / EPAS1 protein, a transcription factor (predicted molecular weight of 97 kDa) induced by hypoxic conditions. It forms a heterodimer with HIF1 beta / ARNT to drive a specific gene expression profile in various physiological and pathological processes that occur in the context of hypoxia.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX30114 Datasheet
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