HIF2 alpha antibody [ep190b]
Catalogue Number: GTX30123-GTX
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WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
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WB analysis of HeLa cell lysate using GTX30123 HIF2 alpha antibody [ep190b].
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WB analysis of multiple samples using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1 and 5: HepG2 without Cobalt (II) Chloride Lane 2 and 6: HepG2 with Cobalt (II) Chloride Lane 3 and 7: HepG2 normoxic Lane 4 and 8 : HepG2 hypoxic Dilution : 1-2 µg/ml
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IHC-P analysis of human heart tissue using GTX30123 HIF2 alpha antibody [ep190b].
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FACS analysis of HepG2 cells using GTX30123 HIF2 alpha antibody [ep190b]. M1 is defined by unstained cells. Purple : primary antibody Dilution : 1:400
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The data was published in the journal PLoS One in 2017. PMID: 29045486
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The data was published in the journal PLoS One in 2008. PMID: 19030229
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 96 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | ep190b |
| Isotype: | IgG1 |
| Immunogen: | Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814] |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC, EMSA |
Description
Description: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
Additional Text
Gene Name
EPAS1
Gene ID
2034
Purification
Protein G purified
Molecular Weight
96
Uniprot ID
Q99814
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1 - 2 µg/ml. IHC-P: 1:150 - 1:300. FCM: 1:400. ELISA: 1:100 - 1:2000. IHC: 1:150 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX30123 Datasheet
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