EP300 antibody [RW105]
Catalogue Number: GTX30618-GTX
GTX30618 Image
GTX30618 Image
GTX30618 Image
GTX30618 Image
GTX30618 Image
FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 2 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
GTX30618 Image
FACS (Intracellular staining) analysis of THP-1 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 1 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
GTX30618 Image
WB analysis of HeLa nuclear extract using GTX30618 EP300 antibody [RW105]. Dilution : 1:250
GTX30618 Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX30618 EP300 antibody [RW105]. Green : Primary antibody Blue : DAPI Permeabilization : 0.05% Triton X-100 in PBS Dilution : 5 µg/mL
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Molecular Weight: | 264 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | RW105 |
| Isotype: | IgG1 |
| Immunogen: | Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947] |
| Application: | FC, ICC, IF, IP, ChIP, EM, WB, InVitroA |
Description
Description: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]
Additional Text
Gene ID
2033
Gene Name
EP300
Uniprot ID
Q09472
Purification
Protein G purified
Molecular Weight
264
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:250 - 1:500. ICC/IF: 1:50 - 1:200. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX30618 Datasheet
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