MAP2 antibody
Catalogue Number: GTX30663-GTX
GTX30663 Image
GTX30663 Image
GTX30663 Image
WB analysis of bovine brain tissue lysate using GTX30663 MAP2 antibody.
GTX30663 Image
ICC/IF analysis of mixed neuron/glia cell cultures using GTX30663 MAP2 antibody. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative.
| Manufacturer: | GeneTex |
| Preservative: | 5 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Purified MAP2 isolated from bovine brain. |
| Application: | ICC, IF, WB, IHC-Fr, IHC |
Description
Description: Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons.
Additional Text
Purification
Ammonium Sulfate Precipitated
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:10000 - 1:20000. ICC/IF: 1:5000 - 1:10000. IHC-Fr: 1:1000 - 1:5000. IHC: 1:1000 - 1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX30663 Datasheet
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