Nestin antibody [10C2]
Catalogue Number: GTX30671-GTX
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IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2]. Dilution : 1:500
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WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Loading : 5µg Dilution : 1:1000
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ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2]. Green : primary antibody(left) and isotype control(right) Blue : DAPI Dilution : 1:50 Permibilization : 0.1% Triton X-100
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FACS (Intracellular staining) analysis of 1 x 10? CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2]. Blue : Primary antibody Orange : isotype control Dilution : 1 µg/10? cells
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The data was published in the journal PLoS One in 2015.PMID: 26720151
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Molecular Weight: | 177 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | nestin , Nbla00170 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 10C2 |
| Isotype: | IgG1 |
| Immunogen: | A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681] |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr, MS |
Description
Description: Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Additional Text
Gene Name
NES
Gene ID
10763
Purification
Protein G purified
Concentration
2.4 mg/mL
Molecular Weight
177
Uniprot ID
P48681
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:1000 - 1:5000. ICC/IF: 1:50 - 1:200. IHC-P: 1:100 - 1:200. IHC-Fr: 1:100 - 1:200. FCM: 1 µg / 10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX30671 Datasheet
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