Streptavidin (HRP)

Catalogue Number: GTX30949-GTX

Manufacturer:	GeneTex
Preservative:	0.05% ProClin 300\|0.05% ProClin 300
Physical state:	Liquid
Type:	Avidin/Streptavidin Biotin Products
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	1 mg
Application:	ELISA, WB, IHC

Overview
Datasheets

Description

Description: Streptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Short Description

Streptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Protein content: > 95%. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C.

GTX30949 Datasheet

GTX30949 Reference

Price

£206.00

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