Trident 10X TBST

Catalogue Number: GTX30976-GTX

Manufacturer:GeneTex
Physical state:Liquid
Type:Detergents
Shipping Condition:RT
Storage Condition:RT
Unit(s): 100 ml
Application: ELISA, WB

Description

Description: This buffer is supplied as 10X TBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. This buffer does not contain any proteins.

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8C for several weeks. Wash membrane or ELISA plates for 10 minutes each for 3 times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.

Short Description

Tris-Buffered Saline with Tween Solution (10X)