PD-L1 antibody
Catalogue Number: GTX31308-GTX
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IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 20 µg/ml
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FACS analysis of A-20 cells using GTX31308 PD-L1 antibody. Working concentration : 0.5 µg/ml Green: Isotype control Red : PD-L1 antibody
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IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody. Red : Primary antibody Dilution : 1:250
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IHC-P analysis of rat heart tissue using GTX31308 PD-L1 antibody. Antigen retrieval : Heat mediation with a citrate buffer (pH6) Dilution : 5 µg/ml
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WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or PD-L1 siRNAs (lane 2) using GTX31308 PD-L1 antibody. Loading : 10µg of lysates per lane Dilution : 2 µg/ml
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WB analysis of PD-L1 overexpression in 293 cells using GTX31308 PD-L1 antibody. Lane 1 : non-transfected 293 cells Lane 2 : PD-L1 overexpressed 293 cells Loading : 15µg of lysates per lane Dilution : 1 µg/ml
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WB analysis of various sample lysates using GTX31308 PD-L1 antibody. Loading : 15µg of lysates per lane Dilution : 2 µg/ml
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WB analysis of Raji cell lysate using GTX31308 PD-L1 antibody. Working concentration : (A) 0.5 and (B) 1 µg/ml
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IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 2.5 µg/ml
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IHC-P analysis of human tonsil tissue using GTX31308 PD-L1 antibody. Working concentration : 5 µg/ml
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The data was published in the journal Nat Commun in 2020.PMID: 32245950
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The data was published in the journal Nat Commun in 2020.PMID: 32245950
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | PD-L1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1.The immunogen is located within amino acids 60 - 110 of PD-L1(Accession number: NP_054862). |
| Application: | ELISA, FC, IHC-P, WB, IHC |
Additional Text
Gene Name
CD274
Gene ID
29126
Purification
Affinity Purified
Uniprot ID
Q9NZQ7
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 0.5 - 1 µg/mL. IHC-P: 2.5 µg/mL. FCM: 0.5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX31308 Datasheet
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