IDH2 antibody
Catalogue Number: GTX32666-GTX
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GTX32666 Image
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ChIP analysis of 293T cell lysate using GTX32666 IDH2 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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IP analysis of MCF-7 cell lysate using GTX32666 IDH2 antibody. Antibody amount : 1µg / 200µg lysate Dilution : 1:1000
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WB analysis of various sample lysates using GTX32666 IDH2 antibody. Dilution : 1:1000 Loading : 25µg per lane
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ICC/IF analysis of L929 cells using GTX32666 IDH2 antibody. Blue : DAPI Dilution : 1:100
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ICC/IF analysis of C6 cells using GTX32666 IDH2 antibody. Blue : DAPI Dilution : 1:100
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IHC-P analysis of human colon carcinoma tissue using GTX32666 IDH2 antibody. Dilution : 1:100
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 51 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2). |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Additional Text
Gene ID
3418
Gene Name
IDH2
Uniprot ID
P48735
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
51
Application Notes
WB: 1:500 - 1:2000. ICC/IF: 1:20 - 1:50. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. ChIP assay: 1:20 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX32666 Datasheet
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