EMI1 antibody [EMI1/1176]

Catalogue Number: GTX34689-GTX

Manufacturer:GeneTex
Preservative:0.05% Sodium azide|0.05% Sodium azide
Molecular Weight:50
Physical state:Liquid
Type:Monoclonal Primary Antibody - Unconjugated
Alias:EMI1 , FBX5 , FBXO5 , Fbox protein 5 , Fbxo31 , Emi1 , F-box protein 5
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: EMI1/1176
Isotype: IgG2a
Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Application: IHC-P, WB, PA

Description

Description: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Additional Text

Gene Name

FBXO5

Purification

Protein A/G Purified

Gene ID

26271

Concentration

0.2 mg/ml

Molecular Weight

50

Uniprot ID

Q9UKT4

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

WB: 1-2µg/ml for 60 minutes at RT. IHC-P: 1-2µg/ml for 30 minutes at RT. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.