CaMKII (oxidized) antibody
Catalogue Number: GTX36254-GTX
GTX36254 Image
GTX36254 Image
GTX36254 Image
GTX36254 Image
GTX36254 Image
GTX36254 Image
Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.
GTX36254 Image
Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.
GTX36254 Image
Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII (GTX36254).
GTX36254 Image
Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.
GTX36254 Image
WB analysis of various sample lysates using using GTX36254 CaMKII (oxidized) antibody. Lane 1 : Recombinant CaMKII Lane 2 : Untreated mouse heart cell lysates Lane 3 : H2O2-treated mouse heart cell lysates Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CAMK , CAMK-II , CAMKG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | CaMKII synthetic peptide with oxidized Met281/282 |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, PLA |
Description
Description: Calcium/calmodulin-dependent protein kinase II (CaMKII) couples increases in Ca2+ to fundamental responses in excitable cells including activation of ion channels, gene transcription and apoptosis. CaMKII is activated by angiotensin II-induced oxidation, which leads to apoptosis in cardiomyocytes. CaMKII activity is also enhanced by pro-oxidant conditions, and the oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM.
Additional Text
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
For WB, use at a dilution of 1:1,000, For IF, use at a dilution of 1:50 with paraffin-embedded sections and 1:100 with frozen sections.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX36254 Datasheet
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