Methylated cytosine DNA standard kit
Catalogue Number: GTX400004-GTX
GTX400004 Image
GTX400004 Image
GTX400004 Image
GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies. The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500
GTX400004 Image
All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.
| Manufacturer: | GeneTex |
| Preservative: | No Preservative |
| Physical state: | Lyophilized |
| Type: | Standards |
| Alias: | 5-methylcytosine , 5-hydroxymethylcytosine , 5-formylcytosine , 5-carboxylcytosine |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 kit |
| Application: | DB |
Additional Text
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
The Methylated cytosine DNA standard kit is a set of five DNA standards that are linear dsDNA, 426 bp, and have the same sequence (see sequence below & figure). The only difference is that each contains either normal cytosines, 5-methylcytosines, 5-hydroxymethylcytosines, 5-formylcytosines, or 5-carboxylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine mod
GTX400004 Datasheet
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