CD3 epsilon antibody [CD3-12]
Catalogue Number: GTX42110-GTX
GTX42110 Image
GTX42110 Image
GTX42110 Image
GTX42110 Image
IHC-P analysis of human tonsil tissue using GTX42110 CD3 antibody. Heat mediated antigen retrieval using EDTA buffer pH9.0.
GTX42110 Image
WB analysis of Jurkat cell lysate using GTX42110 CD3 antibody [CD3-12].
GTX42110 Image
FACS analysis of red cell lysed human peripheral blood gated on leukocytes using GTX42110 CD3 antibody [CD3-12]. Figure A : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat IgG1 isotype control. Figure B : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat anti Human CD3 (GTX42110).
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 23 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | CD3e molecule , IMD18 , T3E , TCRE |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 ug |
| Host name: | Rat |
| Clone: | CD3-12 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC) |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
CD3E
Gene ID
916
Uniprot ID
P07766
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
23
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
IHC-P: 1/100. IHC-Fr: 1/100. FCM: 1/50-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX42110 Datasheet
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