p73 antibody [5B429]
Catalogue Number: GTX46123-GTX
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FACS (Intracellular staining) analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Blue : Primary antibody Orange : isotype control Dilution : 1 µg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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ICC/IF analysis of HeLa cells using GTX46123 p73 antibody [5B429]. Red : primary antibody Blue : DAPI Dilution : 3 ug/mL
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WB analysis of (A)HeLa overexpressing p73 (B) HeLa mock cell lysate using GTX46123 p73 antibody [5B429]. Dilution : 2 µg/ml
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WB analysis of HeLa cell lysate using GTX46123 p73 antibody [5B429].
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IHC-P analysis of human breast carcinoma tissue using GTX46123 p73 antibody [5B429]. Dilution : 5 µg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Alias: | tumor protein p73 , P73 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 5B429 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53. |
| Application: | FACS, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, MS |
Description
Description: This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq]
Additional Text
Concentration
1 mg/ml
Gene ID
7161
Gene Name
TP73
Uniprot ID
O15350
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
70
Application Notes
WB: 2 µg/ml. ICC/IF: 1:20 - 1:1000. IHC-P: 5 - 15 µg/ml. IHC-Fr: 5 - 15 µg/ml. FACS: 1 µg/ml. IP: 1:20 - 1:1000. ChIP assay: 1:20 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX46123 Datasheet
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