MIF antibody
Catalogue Number: GTX48478-GTX
GTX48478 Image
GTX48478 Image
GTX48478 Image
Western blot analysis of GeneTex's IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 µg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
GTX48478 Image
WB analysis of various samples using GTX48478 MIF antibody. Lane 1 : Mock Lane 2 : recombinant human MIF protein Loading : 100 ng
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 12 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | macrophage migration inhibitory factor , GIF , GLIF , MMIF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to KLH. |
| Application: | ELISA, WB |
Description
Description: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Additional Text
Gene Name
MIF
Uniprot ID
P14174
Gene ID
4282
Concentration
5 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
12
Application Notes
WB: 1:500-1:2000. ELISA: 1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48478 Datasheet
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