RNF2 antibody
Catalogue Number: GTX48486-GTX
GTX48486 Image
GTX48486 Image
GTX48486 Image
GTX48486 Image
Western blot using GeneTex's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
GTX48486 Image
Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25oC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25oC. Secondary antibody: Alexa FluorR488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
GTX48486 Image
WB analysis of various samples using GTX48486 RNF2 antibody. Lane 1 : NIH-3T3 whole cell lysate Lane 2 : U937 whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : Mouse brain tissue lysate Lane 5 : CHO-K1 whole cell lysate Loading : 20 µg Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 38 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ring finger protein 2 , BAP-1 , BAP1 , DING , HIPI3 , RING1B , RING2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein. |
| Application: | ELISA, ICC, IF, WB, Multiplex |
Description
Description: RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.
Additional Text
Gene Name
RNF2
Uniprot ID
Q99496
Concentration
1.1 mg/ml
Purification
Affinity Purified
Gene ID
6045
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
38
Application Notes
WB: 1:500-1:2000. ICC/IF: 1:300. ELISA: 1:5000-1:25000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48486 Datasheet
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