DNMT1 antibody [60B1220.1]
Catalogue Number: GTX48548-GTX
GTX48548 Image
GTX48548 Image
GTX48548 Image
GTX48548 Image
GTX48548 Image
GTX48548 Image
FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA
GTX48548 Image
WB analysis of 2102EP cells / human DNMT1 recombinant protein / NIH3T3 cells / D3 cells in the (A)/(C)/(E)/(G) absence and (B)/(D)/(F)/(H) presence of immunizing peptide using GTX48548 DNMT1 antibody [60B1220.1]. Dilution : 5 µg/ml for cells / 0.1µg/ml for recombinant protein
GTX48548 Image
WB analysis of HeLa cell lysate using GTX48548 DNMT1 antibody [60B1220.1].
GTX48548 Image
ICC/IF analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 1:10
GTX48548 Image
IHC-P analysis of human hepatocellular carcinoma tissue using GTX48548 DNMT1 antibody [60B1220.1]. Dilution : 1:50
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 183 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 60B1220.1 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). |
| Application: | FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Flt, MS |
Description
Description: DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
DNMT1
Purification
Protein G purified
Gene ID
1786
Molecular Weight
183
Uniprot ID
P26358
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 0.1 - 0.5 µg/ml. ICC/IF: 1:10. IHC-P: 1:50 - 1:500. IP: 1:10 - 1:500. ChIP assay: 1:20 - 1:1000. IHC (Free Floating): 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48548 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations