STAT5A (phospho Tyr694) antibody [5F6.F1]
Catalogue Number: GTX48648-GTX
GTX48648 Image
GTX48648 Image
GTX48648 Image
GTX48648 Image
GTX48648 Image
GTX48648 Image
Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody(1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.
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Immunohistochemistry using GeneTex's Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Western blot using GeneTex's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4o C. For detection DyLight800 conjugated Goat anti Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature.
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ICC/IF analysis of NIH-3T3 cells using GTX48648 STAT5A (phospho Tyr694) antibody [5F6.F1]. Red : Primary antibody Green : Tubulin Dilution : 1:500
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WB analysis of IL-2 treated (lane 2) and untreated (lane 1) NK92 whole cell lysate using GTX48648 STAT5A (phospho Tyr694) antibody [5F6.F1]. Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 91 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | signal transducer and activator of transcription 5A , AA959963 , STAT5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 5F6.F1 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein. |
| Application: | ELISA, ICC, IF, IHC-P, WB, Multiplex |
Description
Description: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5s. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
Additional Text
Gene Name
STAT5A
Uniprot ID
P42230
Modification
Phosphorylated, Tyr694
Gene ID
20850
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
91
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:2000. ICC/IF: 1:50-1:1000. IHC-P: 20-40 µg/mL. ELISA: 1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48648 Datasheet
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