AKT antibody
Catalogue Number: GTX48651-GTX
GTX48651 Image
GTX48651 Image
GTX48651 Image
GTX48651 Image
Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4o C. IRDYE800 conjugated Dnky-anti-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature.
GTX48651 Image
WB analysis of various samples using GTX48651 AKT antibody. Lane 1 : HeLa whole cell lysate Lane 2 : H2O2-treated MCF-7 whole cell lysate Lane 3 : Recombinant AKT protein Lane 4 : A431 whole cell lysate Loading : 35 µg Dilution : 1:300
GTX48651 Image
WB analysis of various samples using GTX48651 AKT antibody. Lane 1 : Protein ladder Lane 2 : Recombinant AKT1 protein Lane 3 : Recombinant AKT2 protein Lane 3 : Recombinant AKT3 protein Loading : 50 ng Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.1% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region of human AKT1 protein. |
| Application: | ELISA, FC, ICC, IF, WB, IHC |
Description
Description: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability.Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Additional Text
Concentration
0.9 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:2000. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48651 Datasheet
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