IL32A antibody (Biotin)
Catalogue Number: GTX48653-GTX
GTX48653 Image
GTX48653 Image
Western blot using GeneTex's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 27 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | interleukin 32 , IL-32alpha , IL-32beta , IL-32delta , IL-32gamma , NK4 , TAIF , TAIFa , TAIFb , TAIFc , TAIFd |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length recombinant human IL-32A protein. |
| Application: | ELISA, WB, IHC |
Description
Description: IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Additional Text
Gene Name
IL32
Gene ID
9235
Uniprot ID
P24001
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
27
Concentration
1 mg/ml
Application Notes
WB: 1:2000-1:10000. ELISA: 1:20000-1:100000. IHC: 1:1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48653 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations