TAF1 antibody
Catalogue Number: GTX48659-GTX
GTX48659 Image
GTX48659 Image
GTX48659 Image
GTX48659 Image
Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 µg of TAF1 antibody(GTX48659) and an RNA PolII antibody.
GTX48659 Image
Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 µg of TAF1 antibody(GTX48659) and an RNA PolII antibody.
GTX48659 Image
WB analysis of various samples using GTX48659 TAF1 antibody. Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : MCF-7 whole cell lysate Lane 4 : Jurkat whole cell lysate Lane 5 : A431 whole cell lysate Lane 6 : A549 whole cell lysate Lane 7 : LNCap whole cell lysate Lane 8 : Molt-4 whole cell lysate Lane 9 : Ramos whole cell lysate Lane 10 : Raji whole cell lysate Lane 11 : A-172 whole cell lysate Lane 12 : NIH-3T3 whole cell lysate Loading : 35 µg Dilution : 0.2 µg/mL
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 213 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | TATA-box binding protein associated factor 1 , BA2R , CCG1 , CCGS , DYT3 , DYT3/TAF1 , KAT4 , MRXS33 , N-TAF1 , NSCL2 , OF , P250 , TAF(II)250 , TAF2A , TAFII-250 , TAFII250 , XDP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1. |
| Application: | ELISA, IHC-P, IP, ChIP, WB |
Description
Description: Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.
Additional Text
Gene ID
6872
Gene Name
TAF1
Uniprot ID
P21675
Concentration
1.1 mg/ml
Purification
Affinity Purified
Molecular Weight
213
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:100-1:2000. IHC-P: 10 µg/mL. ELISA: 1:150000-1:300000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48659 Datasheet
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